CD45 Anticuerpo (I3/2.3) recomendado para detectar CD45 de mouse origen, mediante WB, IP, IF y FCM

CD45 Anticuerpo (I3/2.3): sc-52491

CD45 Anticuerpo (I3/2.3) clasificado 4.3 de 5 por 4.
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Ficha técnica

    • CD45 Anticuerpo I3/2.3 es un monoclonal de rata IgG2b, ver las 8 publicaciones, proporcionado 200 µg/ml
    • recomendado para detectar CD45 de mouse origen, mediante WB, IP, IF y FCM
    • disponible conjugado tanto a ficoeritrina como a FITC para IF, IHC(P) y FCM
    • Ver CD45 (35-Z6): sc-1178 para CD45 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (I3/2.3). This work is in progress.
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Can CD45 (I3/2.3): sc-52491 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (I3/2.3): sc-52491 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Works as expected in WB Acceptable WB data for WBBYDP and TK-1 whole cell lysates at a 1: 200 dilution.
Fecha de publicación: 2017-02-01
Rated 4 de 5 por de Used this in IF and saw positive signal in Used this in IF and saw positive signal in mouse cells.
Fecha de publicación: 2015-09-23
Rated 4 de 5 por de Published using this antibody for immunofluorescence Published using this antibody for immunofluorescence microscopy. -SCBT Publication Review
Fecha de publicación: 2015-01-14
Rated 4 de 5 por de Produced wonderful Indirect FCM data for Produced wonderful Indirect FCM data for mouse peripheral blood leukocytes stained with CD45 . -SCBT QC
Fecha de publicación: 2013-09-27
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