CD45 Anticuerpo (F10-89-4) recomendado para detectarCD45 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM

CD45 Anticuerpo (F10-89-4): sc-52490

CD45 Anticuerpo (F10-89-4) clasificado 3.8 de 5 por 5.
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Ficha técnica

    • CD45 Anticuerpo (F10-89-4) es un monoclonal de ratón IgG2a κ, ver las 1 publicaciones, proporcionado como 200 µg/ml
    • raised against T lymphocytes of human origin
    • recomendado para detectarCD45 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM
    • disponible conjugado tanto a ficoeritrina como a FITC para IF, IHC(P) y FCM
    • Ver CD45 (35-Z6): sc-1178 para CD45 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD45 Antibody (F10-89-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (F10-89-4) (see ordering information below).
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Can CD45 (F10-89-4): sc-52490 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (F10-89-4): sc-52490 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 4 de 5 por de Good Indirect FCM data of human peripheral Good Indirect FCM data of human peripheral blood leukocytes stained with CD45 . -SCBT QC
Fecha de publicación: 2015-06-01
Rated 3 de 5 por de Faint band present at expected MW in western Faint band present at expected MW in western blot analysis of CD45 expression in human PBL whole cell lysate. -SCBT QC
Fecha de publicación: 2015-05-06
Rated 4 de 5 por de Clean bands in WB with GA Clean bands in WB with GA-10 and Ramos whole cell lysates. -SCBT QC
Fecha de publicación: 2015-02-22
Rated 4 de 5 por de Published IF data in rat MSCs Published IF data in rat MSCs. -SCBT Publication Review
Fecha de publicación: 2015-02-04
Rated 4 de 5 por de Clear FCM data of Jurkat cells Clear FCM data of Jurkat cells. -SCBT QC
Fecha de publicación: 2014-08-22
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