CD45 Anticuerpo (35-Z6) recomendado para detectarCD45 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM

CD45 Anticuerpo (35-Z6): sc-1178

CD45 Anticuerpo (35-Z6) clasificado 4.3 de 5 por 27.
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Ficha técnica

    • Anti-CD45 Anticuerpo 35-Z6 es un monoclonal de ratón IgG1 κ CD45 Anticuerpo, ver las 66 publicaciones, proporcionado como 200 µg/ml
    • raised against the extracellular domain of CD45 of human origin
    • CD45 Anticuerpo (35-Z6) es recomendado para detectar CD45 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM
    • CD45 Anticuerpo (35-Z6) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • también disponibles conjugados a Alexa Fluor® 405 para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de CD45 (35-Z6): sc-1178.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD45 Antibody (35-Z6) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (35-Z6) (see ordering information below).
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Does this antibody detect WBC from C57Bl/6 mice?

Preguntado por: frneubh1
Thank you for your question. Anti-CD45 Antibody (35-Z6) is recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
Respondida por: Tech Support Europe
Fecha de publicación: 2021-04-14

If I am going to stain mouse skin tissues, do I need additional kit, for example "mouse on mouse kit" (as this antibody is mouse origin)?

Preguntado por: hpark
Thank you for your question. It depends on what type of tissues you are staining. If you are staining mouse skin tissues, and there is not a lot of endogenous mouse IgG, then it is probably not necessary to use a special "mouse on mouse kit" for staining. We do recommend including a negative control tissue where you omit primary antibody and stain with secondary antibody alone. Then you can identify any possible non-specific binding from your anti-mouse IgG secondary antibody alone. As an alternative, you could also consider using one of the directly conjugated forms available of this CD45 antibody. Then no secondary antibody is needed for detection, and you can avoid any possible problem of non-specific binding from anti-mouse IgG secondary antibodies.
Respondida por: Technical Support
Fecha de publicación: 2020-05-18

What could be the expiry date for CD45 since we have observed it to be active even after 5 years

Preguntado por: Samarth
Thank you for your question. We do not give our antibodies an expiration date since they can work for up to 10-15 years in some cases. We warranty the performance of our antibody reagents for 1 year from the date of purchase.
Respondida por: Tech Service
Fecha de publicación: 2020-02-26

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CD45 Antibody (35-Z6): sc-1178?

Preguntado por: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Support 6
Fecha de publicación: 2017-01-09
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Rated 5 de 5 por de Excellent antibody for IF We use the antibody to stain T cells in a coculture assays with patient derived prostate cancer organoids. 1:400 dilution in 1% BSA (shown in red).
Fecha de publicación: 2021-03-11
Rated 5 de 5 por de Great CD45 Ab for DAB immunohistochemistry The Ab gives strong and clean signal/noise staining of CD45 in FFPE colon tissue from mouse
Fecha de publicación: 2019-09-20
Rated 5 de 5 por de immunofluorescent staining on immunofluorescent staining this sc-1178 CD45 antibody worked well.
Fecha de publicación: 2019-04-16
Rated 4 de 5 por de Consistent Quality We continue to use this antibody for IF and flow, though have not had success with IP.
Fecha de publicación: 2019-03-18
Rated 1 de 5 por de Bad result on mouse samples Bad result on mouse bone marrow paraffin samples. Few cells stained.
Fecha de publicación: 2018-03-15
Rated 4 de 5 por de Good for Rat and Mouse Received this as a sample. We ran 3 species (H, M, R) on 3 separate test runs. HIER at high pH 9.0 is necessary. The mouse and rat spleens show strong results. The human tissues were very weak, tried both normal and abnormal tissues with same results. We used a concentration of 1:100. If you are running human tissue, I would suggest using a higher concentration than ours.
Fecha de publicación: 2017-12-20
Rated 4 de 5 por de Overall Great! Antibody worked well for IF staining of human T cells at a 1:100 dilution. Also good for flow at 1ug/1 million cells. Western blot seemed good/relatively clean. Did not work for IP at a concentration of 1ug/IP.
Fecha de publicación: 2017-11-20
Rated 4 de 5 por de mouse anti-CD45 antibody I got a free sample and tried on IHC-P. It worked ok, but not very strong.
Fecha de publicación: 2017-09-21
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