CD4 Antibody (W3/25): sc-20079

CD4 Antibody (W3/25) is a mouse monoclonal IgG1 kappa light chain antibody that targets CD4, also known as T4 or Leu 3, in both mouse and rat species. Raised against rat thymocyte membranes, anti-CD4 antibody (W3/25) is recommended for use in immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM), ensuring reliable detection of CD4 in various experimental settings. CD4 is a membrane-bound glycoprotein expressed primarily on T helper cells, as well as on cortical cells, mature medullary thymocytes, microglial cells, and dendritic cells. Functionally, CD4 acts as a co-receptor for the T cell receptor (TCR), enhancing the interaction between TCRs and class II major histocompatibility complex (MHC) molecules on antigen-presenting cells, which is essential for effective T cell activation and the initiation of adaptive immune responses. Additionally, CD4 plays a crucial role as the primary receptor for human immunodeficiency virus (HIV), facilitating viral entry into host cells and contributing to immune system impairment in HIV-infected individuals. The interaction of CD4 with intracellular proteins such as the tyrosine kinase Lck is vital for the transmission of activation signals that drive T cell proliferation and differentiation. By enabling precise identification and characterization of CD4-expressing cells, CD4 (W3/25) monoclonal antibody supports important research in immunology, including studies on autoimmune diseases, infectious diseases, and the development of immunotherapies.

The preferred CD4 antibody is CD4 Antibody (GK1.5): sc-13573.