CD1B3 Antibody (CC122): sc-101831
- CD1B3 Antibody (CC122) is a mouse monoclonal mouse IgG1 κ κ provided at 200 µg/ml
- raised against thymocytes of bovine origin
- Anti-CD1B3 Antibody (CC122) is recommended for detection of CD1B3 of human, bovine and ovine origin by WB, IP and FCM
- Anti-CD1B3 Antibody (CC122) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for CD1B3 Antibody (CC122) for WB applications. This reagent is now offered in a bundle with CD1B3 Antibody (CC122) (see ordering information below).
CD1B3 Antibody (CC122) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD1B3 protein of human, bovine, and ovine origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). CD1B3 (CC122) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The CD1 multigene family encodes five distinct forms of CD1 T-cell surface glycoprotein in humans: CD1A, CD1B, CD1C, CD1D, and CD1E, which play a crucial role in immune response by presenting lipid antigens to T lymphocytes. CD1B3, a 333 amino acid member of this family, is a type 1 membrane protein sharing structural similarities with MHC class I antigens, facilitating lipid-based antigen recognition. CD1B3 interacts with β-2-Microglobulin and expresses on various immune cells, including cortical thymocytes, Langerhans cells, a subset of B cells, and certain dendritic cells. These interactions are vital for CD1-restricted T cell development and activation, as well as proper CD1 molecule trafficking, regulated by adaptor-protein complexes and CD1-associated chaperones. Constitutive endocytosis of CD1B molecules and differential sorting of MHC class II from lysosomes during dendritic cell maturation ensure effective separation of peptide- and lipid antigen-presenting pathways, highlighting CD1B3′s critical role in immune system functionality.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD1B3 Antibody (CC122) References:
- Characterization of guinea-pig group 1 CD1 proteins. | Hiromatsu, K., et al. 2002. Immunology. 106: 159-72. PMID: 12047745
- The bovine CD1 family contains group 1 CD1 proteins, but no functional CD1d. | Van Rhijn, I., et al. 2006. J Immunol. 176: 4888-93. PMID: 16585584
- Crystal structure of bovine CD1b3 with endogenously bound ligands. | Girardi, E., et al. 2010. J Immunol. 185: 376-86. PMID: 20519644
- Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c. | Martin, LH., et al. 1987. Proc Natl Acad Sci U S A. 84: 9189-93. PMID: 2447586
- Expression of cDNA clones encoding the thymocyte antigens CD1a, b, c demonstrates a hierarchy of exclusion in fibroblasts. | Aruffo, A. and Seed, B. 1989. J Immunol. 143: 1723-30. PMID: 2701945
- Molecular cloning of CD1a (T6), a human epidermal dendritic cell marker related to class I MHC molecules. | Longley, J., et al. 1989. J Invest Dermatol. 92: 628-31. PMID: 2784820
- Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to Mycobacterium tuberculosis infection. | Harris, MC., et al. 2023. bioRxiv.. PMID: 37292852
- Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to Mycobacterium tuberculosis infection. | Harris, MC., et al. 2024. Infect Immun. 92: e0038024. PMID: 39494875
- The CD1 family: a third lineage of antigen-presenting molecules. | Porcelli, SA. 1995. Adv Immunol. 59: 1-98. PMID: 7484459
- Surface antigens of human thymocyte populations defined by CD3, CD4 and CD8 expression: CD1a is expressed by mature thymocytes but not peripheral T cells. | Sotzik, F., et al. 1993. Immunol Lett. 36: 101-6. PMID: 7688345
- Class I-like CD1A-C do not protect target cells from NK-mediated cytolysis. | Storkus, WJ., et al. 1996. Cell Immunol. 167: 154-6. PMID: 8548840
- Antigen presentation by CD1 and MHC-encoded class I-like molecules. | Melián, A., et al. 1996. Curr Opin Immunol. 8: 82-8. PMID: 8729450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD1B3 Antibody (CC122) | sc-101831 | 200 µg/ml | $322.00 | |||
CD1B3 Antibody (CC122): m-IgG1 BP-HRP Bundle | sc-531656 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD1B3 Antibody (CC122) AC | sc-101831 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD1B3 Antibody (CC122) HRP | sc-101831 HRP | 200 µg/ml | $322.00 | |||
CD1B3 Antibody (CC122) FITC | sc-101831 FITC | 200 µg/ml | $336.00 | |||
CD1B3 Antibody (CC122) PE | sc-101831 PE | 200 µg/ml | $349.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 488 | sc-101831 AF488 | 200 µg/ml | $364.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 546 | sc-101831 AF546 | 200 µg/ml | $364.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 594 | sc-101831 AF594 | 200 µg/ml | $364.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 647 | sc-101831 AF647 | 200 µg/ml | $364.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 680 | sc-101831 AF680 | 200 µg/ml | $364.00 | |||
CD1B3 Antibody (CC122) Alexa Fluor® 790 | sc-101831 AF790 | 200 µg/ml | $364.00 |