CA II Anticuerpo (G-2) recomendado para detectarCA II de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

CA II Anticuerpo (G-2): sc-48351

CA II Anticuerpo (G-2) clasificado 4.8 de 5 por 4.
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Ficha técnica

    • Anti-CA II Anticuerpo G-2 es un monoclonal de ratón IgG2a κ CA II Anticuerpo, ver las 18 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 191-260 de CA II de origen human
    • CA II Anticuerpo (G-2) es recomendado para detectar CA II de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • CA II Anticuerpo (G-2) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de CA II (G-2): sc-48351.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CA II Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with CA II Antibody (G-2) (see ordering information below).
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what is a recomended dilution for sc48351 AF 488 antibody to stain for FACS.

Preguntado por: angry Bird
Thank you for your question. We recommend using 1 µg of fluorochrome-conjugated antibody primary antibody, CA II (G-2): sc-48351 AF488 for each tube of 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. A link to our recommended protocol for flow cytometry is below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Respondida por: Technical Support
Fecha de publicación: 2017-03-21

Can CA II (G-2): sc-48351 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CA II (G-2): sc-48351 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de We used this antibody to visualize differentiated We used this antibody to visualize differentiated enterocytes on paraffin-embedded sections of human colonic epithelia. Signal specificity was great for doing double and triple stainings. Immunohistochemical staining on FFPE sections from human colonic tissue works also very nicely with this antibody.
Fecha de publicación: 2016-05-31
Rated 5 de 5 por de Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Fecha de publicación: 2015-03-21
Rated 4 de 5 por de Shows good detection of endogenous and transfected Shows good detection of endogenous and transfected CA II in Western Blot -SCBT QC
Fecha de publicación: 2015-02-16
Rated 5 de 5 por de Good results seen in IHC with paraffin Good results seen in IHC with paraffin-embedded mouse intestinal epithelium. -SCBT Publication Review
Fecha de publicación: 2015-02-15
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