ATF-1/ATF1 Anticuerpo (C41-5.1) recomendado para detectarATF-1 p35 de mouse, rat y human origen, mediante WB y IP

ATF-1/ATF1 Anticuerpo (C41-5.1): sc-243

ATF-1/ATF1 Anticuerpo (C41-5.1) clasificado 4.8 de 5 por 10.
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Ficha técnica

    • ATF-1/ATF1 Anticuerpo (C41-5.1) es un monoclonal de ratón IgA κ, ver las 104 publicaciones, proporcionado como 200 µg/ml
    • producido contra una proteina recombinante ATF-1 de origen human
    • recomendado para detectarATF-1 p35 de mouse, rat y human origen, mediante WB y IP
    • TransCruz reactivo para Gel Supershift y ChIP (sc-243 X, 200 µg/0.1 ml)
    • Ver ATF-1/ATF1 (25C10G): sc-270 para ATF-1/ATF1 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de ATF-1/ATF1 (C41-5.1): sc-243.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ATF-1/ATF1 Antibody (C41-5.1). This work is in progress.
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When should the TransCruz reagent sc-243 X be used instead of the standard form sc-243?

Preguntado por: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 4 de 5 por de It is fine. Used for WB to detect human cancers. Got the specific band, but too weak. Supersignal ECL is required.
Fecha de publicación: 2020-08-13
Rated 4 de 5 por de Specific for ATF1 in WB I overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 C41-5.1 was specific to ATF1 in WB, as it recognised only GFP-ATF1 protein, but not GFP-CREB or GFP-CREM. Dilution 1:200 worked very well in WB with overexpressed ATF1 in HEK293 cells, but failed to recognize endogenous ATF1 and gave a very strong background signal in rat primary cortical neuron lysates.
Fecha de publicación: 2018-08-23
Rated 5 de 5 por de Great Antibody for WB This antibody is well-cited for a variety of applications including WB, IP, even ChIP. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Fecha de publicación: 2017-01-30
Rated 5 de 5 por de Used this antibody for WB detection and it Used this antibody for WB detection and it worked well.
Fecha de publicación: 2015-10-05
Rated 5 de 5 por de Strong band in WB in HeLa nuclear extract Strong band in WB in HeLa nuclear extract. -SCBT QC
Fecha de publicación: 2015-04-06
Rated 5 de 5 por de Data published for WB Data published for WB, IP, and EMSA in rat cortical neuron. -SCBT Publication Review
Fecha de publicación: 2015-03-31
Rated 5 de 5 por de Published data for WB in mouse RAW264 Published data for WB in mouse RAW264.7 and human THP-1 cell lines. -SCBT Publication Review
Fecha de publicación: 2015-03-27
Rated 5 de 5 por de Publishable data for EMSA in human JEG Publishable data for EMSA in human JEG-3, JAR, Tera-2, and HeLa cells. -SCBT Publication Review
Fecha de publicación: 2015-03-17
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