USP51 CRISPR/Cas9 KO Plasmid (h): sc-414805
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP51 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP51 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP51 CRISPR/Cas9 KO Plasmid (h) | sc-414805 | 20 µg | $397.00 |
Overview
USP51 encodes a ubiquitin-specific protease that participates in deubiquitination, a key regulatory step controlling protein stability, localization, and signaling output. By reversing ubiquitin conjugation, USP51 can influence proteostasis and the dynamics of multiprotein complexes, with downstream effects on cell-cycle progression, DNA damage responses, and stress-adaptive signaling. Altered activity of deubiquitinases is frequently linked to dysregulated ubiquitin–proteasome system function observed in cancer biology and other disorders involving genome instability or aberrant signaling. USP51 is therefore of interest for dissecting ubiquitin-dependent pathway regulation and identifying context-specific dependencies in human cell models.
USP51 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the USP51 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the USP51 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the USP51 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP51 protein expression.
This CRISPR knockout system enables efficient generation of USP51-deficient cell models for investigation of USP51 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting USP51 exon(s) critical for USP51 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple USP51 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP51 CRISPR/Cas9 KO Plasmid (h) and USP51 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the USP51 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP51 HDR Plasmid (h) and USP51 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by USP51 homology arms to support homology-directed repair at defined USP51 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.