Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
S-100A3 CRISPR/Cas9 KO Plasmid (h): sc-405822
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- S-100A3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the S-100A3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: S-100A3 Antibody (A-11): sc-514339
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
S-100A3 CRISPR/Cas9 KO Plasmid (h) | sc-405822 | 20 µg | $397.00 |
Overview
S100A3 encodes S-100A3, a calcium-binding S100 family protein enriched in keratinizing epithelia and hair follicle biology, where it contributes to cytoskeletal organization and cellular differentiation programs. Through Ca²⁺-dependent conformational changes, S-100A3 can participate in protein–protein interactions that influence epithelial structural remodeling and redox-sensitive processes. Dysregulated expression of S100 proteins, including S100A3, has been associated with altered differentiation states, inflammatory signaling, and tumor-associated phenotypes in epithelial tissues. As a result, S100A3 is frequently investigated in studies of epithelial development, stress responses, and disease-relevant changes in tissue architecture.
S-100A3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the S100A3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the S100A3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the S100A3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish S-100A3 protein expression.
This CRISPR knockout system enables efficient generation of S100A3-deficient cell models for investigation of S-100A3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting S100A3 exon(s) critical for S-100A3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple S100A3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by S-100A3 CRISPR/Cas9 KO Plasmid (h) and S-100A3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the S100A3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by S-100A3 HDR Plasmid (h) and S-100A3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by S100A3 homology arms to support homology-directed repair at defined S100A3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.