Neu4 CRISPR/Cas9 KO Plasmid (h): sc-406284
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neu4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neu4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neu4 CRISPR/Cas9 KO Plasmid (h) | sc-406284 | 20 µg | $397.00 |
Overview
NEU4 encodes human neuraminidase 4 (Neu4), a sialidase that removes terminal sialic acids from glycoproteins and glycolipids, thereby modulating glycoconjugate turnover and cell-surface and organellar signaling. Neu4 activity influences lysosomal and mitochondrial sialylation states and can affect processes such as membrane trafficking, autophagy, and metabolic stress responses through altered glycan composition. By reshaping sialylation-dependent interactions, NEU4 contributes to regulation of immune signaling and cell fate decisions in contexts where glycosylation patterns are remodeled. Dysregulated sialidase activity and aberrant sialylation have been linked to inflammatory phenotypes and cancer-associated changes in cell adhesion and invasion, making NEU4 a useful target for mechanistic glyco-biology studies.
Neu4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NEU4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NEU4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NEU4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neu4 protein expression.
This CRISPR knockout system enables efficient generation of NEU4-deficient cell models for investigation of Neu4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NEU4 exon(s) critical for Neu4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NEU4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neu4 CRISPR/Cas9 KO Plasmid (h) and Neu4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NEU4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neu4 HDR Plasmid (h) and Neu4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NEU4 homology arms to support homology-directed repair at defined NEU4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.