Myosin XVIIIA Double Nickase Plasmid (m): sc-436163-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin XVIIIa Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Myosin XVIIIa Double Nickase Plasmid (m) and Myosin XVIIIa Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Myo18a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myosin XVIIIa Antibody (H-10): sc-365328
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin XVIIIA Double Nickase Plasmid (m) | sc-436163-NIC | 20 µg | $410.00 |
Myo18a encodes myosin XVIIIA, an unconventional myosin that links actin cytoskeleton dynamics to intracellular organization and signaling. In mouse cells, myosin XVIIIA contributes to processes such as cell adhesion, migration, and maintenance of cellular architecture by coordinating actin-associated complexes and scaffolding interactions. Dysregulated actin remodeling and cytoskeletal signaling are broadly relevant to phenotypes involving altered motility, polarity, and tissue organization, making Myo18a a useful target for mechanistic studies in these pathways. Because cytoskeletal remodeling interfaces with pathways that influence proliferation and stress responses, perturbing Myo18a can help dissect how actin-dependent structures shape cell behavior in disease-relevant models.
Myosin XVIIIa Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Myo18a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Myo18a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Myo18a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Myo18a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.