Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MYH10 Double Nickase Plasmid (m): sc-429543-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MYH10 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MYH10 Double Nickase Plasmid (m) and MYH10 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Myh10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MYH10 Antibody (A-3): sc-376942
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYH10 Double Nickase Plasmid (m) | sc-429543-NIC | 20 µg | $410.00 | |||
MYH10 Double Nickase Plasmid (m2) | sc-429543-NIC-2 | 20 µg | $410.00 |
Myh10 encodes non-muscle myosin heavy chain IIB (MYH10), a major actin-based motor that generates contractile forces required for cytokinesis, cell migration, and maintenance of tissue architecture. MYH10-driven actomyosin dynamics regulate cell adhesion and polarity through focal adhesion turnover and RhoA/ROCK-dependent cytoskeletal remodeling, coupling mechanical tension to signaling outputs that shape morphogenesis. In mouse systems, MYH10 activity is closely tied to developmental programs and neural and cardiovascular tissue organization, and dysregulation of non-muscle myosin II function has been associated with defects in cell division, altered motility, and aberrant extracellular matrix interactions relevant to disease-relevant phenotypes. These properties make Myh10 a useful node for dissecting mechanotransduction, cytoskeletal organization, and force-dependent regulation of gene expression.
MYH10 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Myh10 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Myh10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Myh10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Myh10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.