Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JMJD4 CRISPR/Cas9 KO Plasmid (h): sc-406639
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the JMJD4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD4 Antibody (D-10): sc-514881
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD4 CRISPR/Cas9 KO Plasmid (h) | sc-406639 | 20 µg | $397.00 |
Overview
JMJD4 encodes a JmjC domain–containing 2-oxoglutarate–dependent oxygenase implicated in post-translational hydroxylation reactions that can influence protein stability and function. JMJD4 activity has been linked to regulation of translation-related processes through modification of ribosomal and RNA-binding factors, with downstream effects on proteostasis and cellular stress responses. By connecting oxygenase chemistry to gene expression control, JMJD4 is studied in pathways involving metabolic sensing, hypoxia-associated signaling, and adaptive remodeling of the translational machinery. Dysregulation of these processes is relevant to proliferative and stress-adapted states, making JMJD4 a useful target for mechanistic studies in cancer biology and related transcriptional/translation control programs.
JMJD4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the JMJD4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the JMJD4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the JMJD4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish JMJD4 protein expression.
This CRISPR knockout system enables efficient generation of JMJD4-deficient cell models for investigation of JMJD4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting JMJD4 exon(s) critical for JMJD4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple JMJD4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by JMJD4 CRISPR/Cas9 KO Plasmid (h) and JMJD4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the JMJD4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by JMJD4 HDR Plasmid (h) and JMJD4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by JMJD4 homology arms to support homology-directed repair at defined JMJD4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.