Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GCN5 Double Nickase Plasmid (m): sc-420512-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCN5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GCN5 Double Nickase Plasmid (m) and GCN5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Kat2a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GCN5 Antibody (A-11): sc-365321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCN5 Double Nickase Plasmid (m) | sc-420512-NIC | 20 µg | $410.00 |
Kat2a encodes GCN5, a lysine acetyltransferase that deposits histone acetylation marks such as H3K9ac and H3K14ac to promote open chromatin and transcriptional activation. In mouse cells, GCN5 functions within SAGA- and ATAC-like coactivator complexes, coordinating RNA polymerase II transcription with chromatin remodeling during cell cycle progression, DNA damage responses, and differentiation programs. Kat2a/GCN5 activity intersects with developmental and hematopoietic gene regulatory networks and influences pathways linked to cell fate control. Dysregulated chromatin acetylation involving GCN5 has been associated with proliferative phenotypes and altered transcriptional states relevant to cancer and neurodevelopmental biology, supporting mechanistic studies in epigenetic regulation.
GCN5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Kat2a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Kat2a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Kat2a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Kat2a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.