CNOT6 Double Nickase Plasmid (h2): sc-406273-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNOT6 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CNOT6 Double Nickase Plasmid (h2) and CNOT6 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting CNOT6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNOT6 Antibody (2193C2a): sc-81231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNOT6 Double Nickase Plasmid (h2) | sc-406273-NIC-2 | 20 µg | $410.00 |
Human CNOT6 encodes the CCR4-NOT complex subunit 6, a cytoplasmic deadenylase that shortens mRNA poly(A) tails to promote transcript destabilization and translational repression, thereby shaping gene expression programs downstream of RNA-binding proteins and microRNA-mediated silencing. As a catalytic component of the CCR4-NOT complex, CNOT6 contributes to post-transcriptional control of cell-cycle progression, differentiation, and stress responses through regulated mRNA turnover and interactions with decapping and decay machinery. Dysregulated CNOT6 activity and CCR4-NOT-dependent RNA homeostasis have been associated with altered proliferative signaling and immune-related gene expression patterns observed in diverse cancers and inflammatory contexts. Gene editing or perturbation of CNOT6 supports mechanistic studies of mRNA decay kinetics, poly(A) tail dynamics, and pathway-level effects on transcriptome stability in human cell models.
CNOT6 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the CNOT6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CNOT6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CNOT6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CNOT6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.