Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Bmx Double Nickase Plasmid (h): sc-401550-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Bmx Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Bmx Double Nickase Plasmid (h) and Bmx Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BMX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Bmx Antibody (C-4): sc-376686
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bmx Double Nickase Plasmid (h) | sc-401550-NIC | 20 µg | $410.00 | |||
Bmx Double Nickase Plasmid (h2) | sc-401550-NIC-2 | 20 µg | $410.00 |
BMX encodes the non-receptor tyrosine kinase Bmx (bone marrow kinase on the X chromosome), a Tec family member that integrates signals from receptor tyrosine kinases, cytokine receptors, and integrins. Bmx contributes to phosphorylation cascades that modulate PI3K–AKT, MAPK/ERK, STAT, and NF-κB-associated processes, influencing cell survival, cytoskeletal dynamics, adhesion, and migration. It is expressed in multiple hematopoietic and endothelial contexts and can shape inflammatory and angiogenic responses through downstream transcriptional programs. Altered BMX activity or expression has been associated with oncogenic signaling networks and tumor microenvironment biology, supporting its study in cancer, vascular biology, and immune signaling models.
Bmx Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BMX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BMX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BMX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BMX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.