alpha-Actin Antikörper (1A4) Empfohlen für die Detektion von smooth muscle α-Actin aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA

α-Actin Antikörper (1A4): sc-32251

α-Actin Antikörper (1A4) wurde bewertet mit 4.7 von 5 von 26.
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Datenblatt

    • Anti-alpha-Actin Antikörper 1A4 ist ein Maus monoklonales IgG2a κ α-Actin Antikörper, verwendet in 317 wissenschaftlichen Veröffentlichungen, in einer Menge von 200 µg/ml
    • raised against an N-terminal decapeptide of α-Actin of human origin
    • Anti-alpha-Actin Antikörper (1A4) ist empfohlen für die Detektion von smooth muscle α-Actin aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA; keine Kreuzreaktivität mit actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
    • Anti-alpha-Actin Antikörper (1A4) ist erhältlich als Konjugat mit Agarose für IP; HRP für WB, IHC(P) und ELISA; und entweder mit Phycoerythrin oder FITC für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 oder Alexa Fluor® 647 für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 680 oder Alexa Fluor® 790 für WB (NIR), IF und FCM
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom α-Actin (1A4): sc-32251 zu erhalten.
    • m-IgG Fc BP-HRP und m-IgG2a BP-HRP sind die empfohlenen Detektionsreagenzien für α-Actin Antikörper (1A4) für WB and IHC(P) Experimente. Diese Reagenzien werden nun auch als Bundle angeboten, zusammen mit α-Actin Antikörper (1A4) (Bestellinformation unten stehend).
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I failed in ELISA with this antibody. Briefly, I coated alpha-actin on Ni plate, applied this antibody HRP, substrate TMB. No color.

Gefragt von: LucyLu
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2020-07-07

Quel est le tampon de démasquage et la concentration utilisé pour un marquage sur coupe paraffine ?

Gefragt von: acertain
Merci pour votre question. Sur coupes en paraffine, nous recommandons d’effectuer un démasquage de l’antigène en tampon citrate. Vous trouverez plus de précisions dans notre page protocoles dans le lien suivant : https://www.scbt.com/scbt/resources/protocols Thank you for your question. In IHC on paraffin embedded tissues sections, we recommend to perform antigen retrieval in citrate buffer. You will find additional information in our protocols in the following link : https://www.scbt.com/scbt/resources/protocols
Beantwortet von: SCBT Heidelberg Support
Veröffentlichungsdatum: 2018-11-27

If I want to perform a Western blot using the α-Actin Antibody (1A4) on human samples, what secondary reagent should I use for detection?

Gefragt von: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Beantwortet von: Tech Service 8
Veröffentlichungsdatum: 2016-12-16

Can I use this mouse monoclonal antibody for triple staining even if my other primary antibodies were also raised in mice?

Gefragt von: Trav11
When looking to perform triple staining with multiple mouse primary antibodies, we recommend using our directly conjugated mouse monoclonal primary antibodies. The α-Actin Antibody (1A4): sc-32251 is currently available conjugated to HRP (sc-32251 HRP), phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647).
Beantwortet von: Tech Service 8
Veröffentlichungsdatum: 2016-12-16

I would like to know whether this antibody is usable to evaluate the expression pattern of alfa-actin protein in adult zebrafish especially skeletal muscles. If not, could you please tell us the other useful antibodies?

Gefragt von: Yosuke
Thank you for your question. α-Actin Antibody (1A4): sc-32251 is recommended for detection of smooth muscle α-Actin, rather than skeletal muscle actin isoforms. This antibody has not yet been tested in zebrafish, and it is unknown if it would work for that species. As a recommended alternative, we would suggest our mouse monoclonal, β-Actin Antibody (C4): sc-47778. This antibody is recommended for detection of β-Actin; may cross-react with all six known isoforms of Actin (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform). This antibody has also been cited in the literature used in zebrafish in the following articles on NCBI's PubMed: PMID: 25075304, 22139372.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2016-12-02
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Rated 5 von 5 von aus very clean background WB recommends the use of 1:1000 dilution, antibody specific binding and strong band appearance. So far, no other experiments have been done.
Veröffentlichungsdatum: 2017-11-01
Rated 1 von 5 von aus Not well work in WB Too many unspecific WB band detected in human liver cancer BEL-7404 lysates. dilution ratio is 1:100
Veröffentlichungsdatum: 2017-10-11
Rated 4 von 5 von aus works good on IF of tissue samples We used this product for the detection of a-SMA in the mouse fibrotic liver frozen sections.
Veröffentlichungsdatum: 2017-10-04
Rated 5 von 5 von aus Good for WB, IF, FACS This antibody was used successfully for immunofluorescence (1:100), Western blot (1:500), and indirect flow cytometry (1:200). Good signal and specificity with very little background.
Veröffentlichungsdatum: 2017-09-14
Rated 5 von 5 von aus Useful antibody! I bought this antibody a year ago and use it in WB analyse. This a-Actin antibody could specificly recognise a-SMA(a-smooth muscle actin).a-SMA is quite important to my study on liver fibrosis and hepatic stellate cells.In my experiment,I use the antibody to analyse a-SMA protein expression in cell extracts and mouse liver tissues.I incubated the PVDF membrane with a-actin antibody in a dilution of 1:1000 for a whole night. It is amazing!
Veröffentlichungsdatum: 2017-09-01
Rated 5 von 5 von aus good antibody This is a trial product, which I was applied for and It's very great !
Veröffentlichungsdatum: 2017-08-26
Rated 5 von 5 von aus good antibody This antibody shows a robust clear band and it is perfect
Veröffentlichungsdatum: 2017-07-25
Rated 5 von 5 von aus WB analysis For mouse brain tissue analysis, there was band detected by a-actin antibody the molecular weight around 43KDa.No background .
Veröffentlichungsdatum: 2017-07-08
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