Ac-DEVD-AFC (CAS 201608-14-2)
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes′ shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.
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SDS (MSDS) herunterladen
SAMPLE Certificate of Analysis (COA)
Ac-DEVD-AFC | CAS 201608-14-2 SAMPLE Certificate of AnalysisAnalysenzertifikat
SAMPLE Certificate of Analysis (COA)
| Test | Specification | Results |
|---|---|---|
| Appearance | White powder | |
| Identification (NMR) | Consistent with structure | |
| Purity (HPLC) | ≥ 98% |
TEST CONDITIONS
Exp. Date: 11/23/2026
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