Whole Cell Lysates for Western Blotting

Santa Cruz Biotechnology offers whole cell lysates that are provided as Western blot positive controls. Each vial contains 500 µg protein in 200 µl SDS-PAGE Western blotting buffer. Recommended use is 50 µg (20 µl) per lane. Lysates should be stored at -20°C and repeated freezing and thawing should be minimized. Sample vial should be boiled once prior to use. Select lysates, as indicated, are treated with alkaline phosphatase (AP) as phosphorylation depleted negative controls for phosphorylation-induction studies.