FAM46A Activators
Chemical activators of FAM46A work through various signaling pathways to promote the phosphorylation and activation of this protein. Phorbol 12-myristate 13-acetate (PMA) is one such activator that directly stimulates protein kinase C (PKC). PKC is a family of enzymes that are pivotal in several cellular processes, and their activation leads to the phosphorylation of a multitude of proteins, including FAM46A. This phosphorylation event alters the functional state of FAM46A, resulting in its activation. Another activator, Forskolin, works through a different mechanism. By elevating intracellular cAMP levels, Forskolin activates protein kinase A (PKA), an enzyme that also phosphorylates FAM46A, triggering its activation. Similarly, Dibutyryl-cAMP, a cAMP analog, bypasses cell membrane restrictions and directly activates PKA, leading to the phosphorylation and activation of FAM46A.
Ionomycin, by increasing intracellular calcium levels, can activate calcium/calmodulin-dependent protein kinases (CaMKs), which may phosphorylate FAM46A, while Sodium Fluoride inhibits protein phosphatases, leading to a sustained phosphorylated and active state of FAM46A. Anisomycin, through its activation of stress-activated protein kinases (SAPKs), can contribute to the phosphorylation landscape of FAM46A, facilitating its activation. Similarly, Okadaic Acid and Calyculin A, both protein phosphatase inhibitors, prevent the dephosphorylation of proteins, indirectly maintaining FAM46A in an activated state. Zinc Chloride and Magnesium Sulfate, serving as cofactors, might enhance the activity of protein kinases, thereby promoting the phosphorylation and activation of FAM46A. Calcium Chloride has a similar effect by increasing intracellular calcium levels, which stimulates calcium-dependent protein kinases that can activate FAM46A. Lastly, although H-89 is typically a kinase inhibitor, its off-target effects can lead to the activation of kinases that are capable of phosphorylating FAM46A, thus contributing to its active state.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA directly activates protein kinase C (PKC) which is known to phosphorylate a wide array of proteins. Given that phosphorylation can activate proteins, including FAM46A, PKC activation by PMA can lead to the phosphorylation and consequent activation of FAM46A. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium/calmodulin-dependent protein kinases (CaMKs). These kinases have the potential to phosphorylate and thereby activate FAM46A. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 and 2A. Inhibition of these phosphatases prevents dephosphorylation of proteins, maintaining them in an active phosphorylated state. Therefore, Okadaic Acid can indirectly maintain the phosphorylation and activation of FAM46A. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a membrane-permeable cAMP analog that activates PKA. Activated PKA can phosphorylate FAM46A, which may result in its activation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates stress-activated protein kinases (SAPKs), which can lead to the phosphorylation of numerous substrates, potentially including FAM46A, hence activating it. | ||||||
Sodium Fluoride | 7681-49-4 | sc-24988A sc-24988 sc-24988B | 5 g 100 g 500 g | $39.00 $45.00 $98.00 | 26 | |
Sodium Fluoride is an inhibitor of protein phosphatases and its action would lead to a reduction in dephosphorylation of proteins, potentially resulting in the sustained activation of FAM46A through its phosphorylated state. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc Chloride can act as a cofactor that stabilizes the structure of proteins and enzymes. By stabilizing protein kinase structures, it may enhance their activity leading to increased phosphorylation and activation of FAM46A. | ||||||
Magnesium sulfate anhydrous | 7487-88-9 | sc-211764 sc-211764A sc-211764B sc-211764C sc-211764D | 500 g 1 kg 2.5 kg 5 kg 10 kg | $45.00 $68.00 $160.00 $240.00 $410.00 | 3 | |
Magnesium Sulfate can serve as a cofactor for various kinases, and its presence is required for the activity of many ATP-utilizing enzymes. Its role in facilitating kinase activity can lead to the phosphorylation and activation of FAM46A. | ||||||
Calcium chloride anhydrous | 10043-52-4 | sc-207392 sc-207392A | 100 g 500 g | $65.00 $262.00 | 1 | |
Calcium Chloride can increase intracellular calcium levels, which may activate calcium-dependent protein kinases that are capable of phosphorylating and activating FAM46A. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatase 1 and 2A like Okadaic Acid and would similarly prevent dephosphorylation of proteins. This inhibition can indirectly result in the phosphorylation and sustained activation of FAM46A. | ||||||