Nuclear Extracts for Gel Shift and Western Blotting

Santa Cruz Biotechnology offers nuclear extracts for gel shift and Western blotting. Nuclear extracts are prepared by the method of Dignam, et al. (1983) Nucleic Acids Res. 11: 1475, and are provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT. Product is supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer. Extracts should be stored at -70° C and repeated freezing and thawing should be avoided.