Santa Cruz Biotechnology offers an expansive library of transfected lysates that overexpress signaling intermediates. Signaling intermediates can respond to a broad range of ligand activated receptor tyrosine kinases where phosphorylation events play a pivotal role. For a well-known example, the Shc gene encodes three widely expressed proteins which act as substrates for receptors and tyrosine kinases in signal transduction pathways. Growth factor receptors with tyrosine kinase activity phosphorylate and thus modulate the function of Shc. Transfected lysates that express signaling intermediates offer a reliable means to evaluate antibody quality and serve as excellent positive controls for experiments involving antibodies.
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