Santa Cruz Biotechnology offers both Complete (CFA) and Incomplete Adjuvant (IFA) formulations of a popular immunopotentiator that are convenient and ready-to-use. The mycobacteria in Complete Freund's adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund's Adjuvant is used for the initial injections; Incomplete Freund's adjuvant is used for the subsequent boosts. Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. Freund's Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.
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