Pax-6 Antibody (PAX6): sc-81649
- Pax-6 Antibody (PAX6) is a mouse monoclonal IgG1 κ Pax-6 antibody, cited in 65 publications, provided at 200 µg/ml
- raised against amino acids 1-223 of Pax-6 of chicken origin
- Pax-6 Antibody (PAX6) is recommended for detection of Pax-6 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
- Anti-Pax-6 Antibody (PAX6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pax-6 Antibody (PAX6) for WB and IHC(P) applications. These reagents are now offered in bundles with Pax-6 Antibody (PAX6) (see ordering information below).
Pax-6 Antibody (PAX6) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Pax-6 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pax-6 antibody (PAX6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-6 plays a crucial role in eye development and is essential for the formation of the eye′s anterior segment, including the cornea and iris. Pax-6 function is vital, as mutations in the Pax-6 gene can lead to severe congenital eye disorders, such as aniridia, characterized by the absence of the iris, which can result in significant vision impairment or blindness. Pax-6 is involved in other anterior segment malformations, including Peters anomaly, which is marked by corneal clouding and various adhesions affecting the eye′s structures. Pax-6 gene encodes a transcriptional regulator that binds to specific DNA sequences through its paired-type DNA-binding domain, which consists of two subdomains that work together to regulate gene expression critical for eye development. Human Pax-6 gene produces two alternatively spliced isoforms, each with distinct structural features that contribute to its regulatory functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pax-6 Antibody (PAX6) References:
- Missense mutation in the alternative splice region of the PAX6 gene in eye anomalies. | Azuma, N., et al. 1999. Am J Hum Genet. 65: 656-63. PMID: 10441571
- Sox-2 is expressed by glial and progenitor cells and Pax-6 is expressed by neuroblasts in the human subventricular zone. | Baer, K., et al. 2007. Exp Neurol. 204: 828-31. PMID: 17291498
- Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless. | Fic, W., et al. 2007. PLoS One. 2: e253. PMID: 17327915
- Protein phosphatase-1 modulates the function of Pax-6, a transcription factor controlling brain and eye development. | Yan, Q., et al. 2007. J Biol Chem. 282: 13954-65. PMID: 17374606
- Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. | Xu, H., et al. 2007. Invest Ophthalmol Vis Sci. 48: 1674-82. PMID: 17389499
- Mutation analysis of gene PAX6 in human gliomas. | Pinto, GR., et al. 2007. Genet Mol Res. 6: 1019-25. PMID: 18273794
- PAX6 analysis of two unrelated families from the Arabian Peninsula with classic hereditary aniridia. | Khan, AO. and Aldahmesh, MA. 2008. Ophthalmic Genet. 29: 145-8. PMID: 18766996
- Expression of pax-6 during urodele eye development and lens regeneration. | Del Rio-Tsonis, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 5092-6. PMID: 7761453
- Mutations at the PAX6 locus are found in heterogeneous anterior segment malformations including Peters' anomaly. | Hanson, IM., et al. 1994. Nat Genet. 6: 168-73. PMID: 8162071
- PAX6 mutations in aniridia. | Hanson, IM., et al. 1993. Hum Mol Genet. 2: 915-20. PMID: 8364574
- Pax-6 and alphaB-crystallin/small heat shock protein gene regulation in the murine lens. Interaction with the lens-specific regions, LSR1 and LSR2. | Gopal-Srivastava, R., et al. 1996. J Biol Chem. 271: 23029-36. PMID: 8798491
- Regulation of PAX-6 gene transcription: alternate promoter usage in human brain. | Okladnova, O., et al. 1998. Brain Res Mol Brain Res. 60: 177-92. PMID: 9757029
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-6 Antibody (PAX6) | sc-81649 | 200 µg/ml | $322.00 | |||
Pax-6 Antibody (PAX6): m-IgG Fc BP-HRP Bundle | sc-528709 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pax-6 Antibody (PAX6): m-IgGκ BP-HRP Bundle | sc-521177 | 200 µg Ab, 40 µg BP | $361.00 | |||
Pax-6 Antibody (PAX6): m-IgG1 BP-HRP Bundle | sc-543080 | 200 µg Ab; 20 µg BP | $361.00 | |||
Pax-6 Antibody (PAX6) AC | sc-81649 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pax-6 Antibody (PAX6) HRP | sc-81649 HRP | 200 µg/ml | $322.00 | |||
Pax-6 Antibody (PAX6) FITC | sc-81649 FITC | 200 µg/ml | $336.00 | |||
Pax-6 Antibody (PAX6) PE | sc-81649 PE | 200 µg/ml | $349.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 488 | sc-81649 AF488 | 200 µg/ml | $364.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 546 | sc-81649 AF546 | 200 µg/ml | $364.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 594 | sc-81649 AF594 | 200 µg/ml | $364.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 647 | sc-81649 AF647 | 200 µg/ml | $364.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 680 | sc-81649 AF680 | 200 µg/ml | $364.00 | |||
Pax-6 Antibody (PAX6) Alexa Fluor® 790 | sc-81649 AF790 | 200 µg/ml | $364.00 |