epitope mapping near the C-terminus of β-1,3-Gal-TL of human origin
recommended for detection of β-1,3-Gal-TL of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
β-1,3-Gal-TL Background Information ∫-1,3-Gal-TL (∫ 1,3-glycosyltransferase-like, B3GTL, B3Glc-T or Gal-T) is a ubiquitously expressed O-fucosyltransferase with highest levels of expression in testis and uterus. It is a single pass type II membrane protein that localizes to the endoplasmic reticulum. ∫-1,3-Gal-TL contributes to O-fucosylglycan elongation on thrombosin Type 1 repeat (TSR) domains. It adds a glucose from UDP-glucose to a particular å-linked fucose in correctly folded TSRs, possibly recognizing a specific fold as opposed to amino acid sequence. ∫-1,3-Gal-TL belongs to the glycosyltransferase 31 family of enzymes. It is conserved from Caenorhabditis elegans to humans and shares 28% homology with Fringe. It contains a DXD motif that is required for its catalytic activity and a KDEL-like REEL sequence at its C-terminal. Mutations in the gene encoding ∫-1,3-Gal-TL can result in Peters Plus syndrome.
