p-EGFR Antibody (12A3): sc-57542

p-EGFR Antibody (12A3) is a mouse monoclonal IgG1 antibody that detects Tyr 845 phosphorylated EGFR in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). p-EGFR monoclonal antibody (12A3) is available in a non-conjugated form, providing researchers with a versatile tool for studying the epidermal growth factor receptor (EGFR), a critical player in cell signaling pathways that regulate cell growth, differentiation, and survival. EGFR functions as a receptor for epidermal growth factors, which, upon binding, activate intrinsic tyrosine kinase activity, leading to autophosphorylation at key tyrosine residues such as Tyr 1092 and Tyr 1173. These phosphorylation events are crucial for recruiting signaling proteins, including the phosphotyrosine binding domain of GRB2, which facilitates downstream signaling through the Ras pathway. Additionally, phosphorylation of these residues induces conformational changes in the C-terminal tail of EGFR, further enhancing signaling capabilities. Understanding EGFR phosphorylation dynamics is vital for elucidating involvement in various cancers and developing targeted therapies. p-EGFR (12A3) antibody serves as an essential resource for investigating EGFR phosphorylation dynamics and impact on cell signaling, providing valuable insights into cancer biology and therapeutic strategies.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-EGFR Antibody (12A3) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-p-EGFR Antibody (12A3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-EGFR Antibody (12A3) Specifications

Alternate Product Name: EGFR

Product Clone Name: 12A3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: Tyr 845

Epitope Target: p-EGFR

Epitope Species: human

Product Reactivity/Detection: Tyr 845 phosphorylated EGFR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for p-EGFR Antibody (12A3): N/A

The preferred EGFR antibody is EGFR Antibody (528): sc-120.