CD45 Antibody (30-F11): sc-53665
- CD45 Antibody (30-F11) is a rat monoclonal IgG2b, cited in 29 publications, provided at 200 µg/ml
- raised against thymus and spleen of mouse origin
- CD45 Antibody (30-F11) is recommended for detection of CD45 of mouse origin by WB, IP, IF, IHC(P) and FCM
- Anti-CD45 Antibody (30-F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (30-F11). This work is in progress.
CD45 Antibody (30-F11) is a rat monoclonal IgG2b light chain antibody developed to recognize CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a crucial transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice, playing a fundamental role in regulating immune cell signaling and activation. Functioning as a tyrosine phosphatase, CD45 modulates key signal transduction pathways by dephosphorylating tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70. These interactions are essential for the proper functioning of T and B cell receptors, influencing lymphocyte development, activation, and overall immune responses. The ability of CD45 to regulate these signaling pathways is critical for maintaining immune system homeostasis and ensuring effective immune responses against pathogens. Anti-CD45 antibody (30-F11) is raised against thymus and spleen tissues of mouse origin, making CD45 (30-F11) antibody effective for various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). By targeting CD45, anti-CD45 antibody (30-F11) facilitates the study of immune cell populations and their activation states, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases. CD45 monoclonal antibody (30-F11) is an indispensable tool for both research and diagnostic purposes in immunology and hematology, supporting advancements in understanding immune responses and the development of targeted therapies for immune disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45 Antibody (30-F11) | sc-53665 | 200 µg/ml | $322.00 | |||
CD45 Antibody (30-F11) AC | sc-53665 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD45 Antibody (30-F11) HRP | sc-53665 HRP | 200 µg/ml | $322.00 | |||
CD45 Antibody (30-F11) FITC | sc-53665 FITC | 200 µg/ml | $336.00 | |||
CD45 Antibody (30-F11) PE | sc-53665 PE | 200 µg/ml | $349.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 488 | sc-53665 AF488 | 200 µg/ml | $364.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 546 | sc-53665 AF546 | 200 µg/ml | $364.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 594 | sc-53665 AF594 | 200 µg/ml | $364.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 647 | sc-53665 AF647 | 200 µg/ml | $364.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 680 | sc-53665 AF680 | 200 µg/ml | $364.00 | |||
CD45 Antibody (30-F11) Alexa Fluor® 790 | sc-53665 AF790 | 200 µg/ml | $364.00 |