epitope mapping near the C-terminus of Testican-3 of human origin
recommended for detection of Testican-3 of mouse, rat and human origin by WB, IF and ELISA; not recommended for the detecion of Testican-3 insoform 2; also reactive with additional species, including canine and porcine
Testican-3 Background Information The Testican family, also designated the BM-40/SPARC/osteonectin family, is composed of highly conserved, extracellular, calcium-binding, sulfate proteoglycans. Expression of Testicans is detected in a variety of tissues, but is most abundant in brain. Family members include Testican-1, Testican-2, Testican-3 and an amino-terminal splice variant of Testican-3, designated N-Tes. Most Testicans inhibit MT-MMPs, thereby inhibiting the activity of pro-MMP-2. Testican-3 is a 436 amino acid protein that contains a follistatin-like domain, a calcium-binding domain, a COOH-terminal thyroglobulin domain and two glycosaminoglycan attachment sites. Both Testican-3 and N-Tes are expressed in normal brain but downregulated in glioma tissues. Transfection of either the N-Tes or Testican-3 gene into transformed glioma or kidney cells suppresses their invasive growth in collagen gel, suggesting that both N-Tes and Testican-3 interfere with tumor invasion.
