epitope corresponding to amino acids 131-230 mapping within an internal region of 12-LO of human origin
recommended for detection of 12-LO of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and canine
12-LO Background Information Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes, the synthesis of signalling molecules as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals, 12-LO and 15-LO, are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. The 12-LO pathway is a regulator of cell survival and apoptosis and affects the expression and localization of the Integrin aV/b5 and Actin microfilaments in rat Walker 256 carcinosarcoma cells. Platelet-type 12-LO regulates the growth and survival of a number of cancer cells. Human platelets metabolize arachidonic acid via 12-lipoxygenase to 12-hydroxyeicosatetraenoic acid.
