epitope mapping within an internal region of GADD 45β of human origin
recommended for detection of GADD 45β of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
GADD 45β Background Information Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively (1). The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45ß and GADD 45g, have been shown to be induced by environmental stresses. GADD 45ß and GADD 45g are thought to induce p38/JNK activation via MEKK4 activation.
