Epac Antibody (A-5): sc-28366

Epac Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Epac protein of mouse, rat, human, and hamster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Epac antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Epac protein, also known as Exchange Protein Directly Activated By cAMP 1, plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor that activates the Ras family GTPases, Rap 1 and Rap 2, in a cAMP-dependent manner. Such activation is vital for mediating various physiological responses, including gene expression, cell growth, differentiation, secretion, and neurotransmission, which are essential for maintaining normal cellular functions and responses to external stimuli. The human Epac gene is located on chromosome 12q13.11, with transcript being highly expressed in the kidney and heart, and in situ hybridization studies have shown expression in the adult rat brain, particularly in regions such as the septum and thalamus during neonatal development. These findings highlight the importance of Epac in both developmental and adult physiological processes, making Epac (A-5) antibody a valuable tool for researchers studying cAMP signaling pathways and their implications in health and disease.

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Epac Antibody (A-5) References:

1. Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs.  |  de Rooij, J., et al. 2000. J Biol Chem. 275: 20829-36. PMID: 10777494
2. Characterization of the gene EPAC2: structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform.  |  Ueno, H., et al. 2001. Genomics. 78: 91-8. PMID: 11707077
3. Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis.  |  Fujimoto, K., et al. 2002. J Biol Chem. 277: 50497-502. PMID: 12401793
4. The role of Epac in the heart.  |  Fujita, T., et al. 2017. Cell Mol Life Sci. 74: 591-606. PMID: 27549789
5. Epac and Nociceptor Sensitization.  |  Huang, LY. and Gu, Y. 2017. Mol Pain. 13: 1744806917716234. PMID: 28580839
6. Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells.  |  Ramos-Alvarez, I., et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316: G263-G277. PMID: 30520694
7. Selective small-molecule EPAC activators.  |  Luchowska-Stańska, U., et al. 2019. Biochem Soc Trans. 47: 1415-1427. PMID: 31671184
8. EPAC in Vascular Smooth Muscle Cells.  |  Wehbe, N., et al. 2020. Int J Mol Sci. 21: PMID: 32708284
9. The Role of Epac in Cancer Progression.  |  Wehbe, N., et al. 2020. Int J Mol Sci. 21: PMID: 32899451
10. Epac as a tractable therapeutic target.  |  Slika, H., et al. 2023. Eur J Pharmacol. 945: 175645. PMID: 36894048
11. cAMP-PKA/EPAC signaling and cancer: the interplay in tumor microenvironment.  |  Zhang, H., et al. 2024. J Hematol Oncol. 17: 5. PMID: 38233872
12. A family of cAMP-binding proteins that directly activate Rap1.  |  Kawasaki, H., et al. 1998. Science. 282: 2275-9. PMID: 9856955

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Epac Antibody (A-5) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Epac Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Epac Antibody (A-5) Specifications

Alternate Product Name: RAPGEF3

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-70

Epitope Target: Epac

Epitope Species: human

Product Reactivity/Detection: Epac

Species Reactivity/Detection: mouse, rat, human, hamster

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Epac Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin