epitope mapping within an internal region of AFAP-110 of human origin
recommended for detection of AFAP-110 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
AFAP-110 Background Information Actin filament associated protein (AFAP-110) interacts directly with Actin filaments through its C-terminal Actin binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. AFAP-110 links signaling molecules to Actin filaments, provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. Deletion of certain binding elements of AFAP-110 results in altered Actin phenotypes; for instance, deletion of the leucine zipper motif causes repositioning of Actin into rosettes. Because inhibition of certain Actin cytoskeletal conformations inhibits cell division and movement, these conformational changes to AFAP-110, and thus Actin organization in the cell, represent a possible therapeutic target for controlling tumorigenesis and metastasis.
AFAP-110 (E-15)
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AFAP-110 (E-15): sc-26271. Western blot analysis of AFAP-110 expression in non-transfected: sc-117752 (A) and human AFAP-110 transfected: sc-114982 (B) 293T whole cell lysates.
