14-3-3 ε Antibody (8C3): sc-23957

14-3-3 ε Antibody (8C3) is a mouse monoclonal IgG2a kappa light chain antibody that detects 14-3-3 epsilon protein of mouse, rat, human, and ovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. 14-3-3 ε monoclonal antibody (8C3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 epsilon protein plays a crucial role in regulating cellular processes such as apoptosis, mitogenic signaling, and cell-cycle checkpoints, making 14-3-3 epsilon significant in cancer biology. 14-3-3 epsilon protein is primarily located in the cytoplasm, where 14-3-3 epsilon interacts with a variety of target proteins, facilitating proper function and localization. Cytoplasmic presence of 14-3-3 proteins is essential for sequestering and regulating ligands, which often contain a 14-3-3 consensus binding motif and are typically phosphorylated on serine or threonine residues. By binding to these phosphorylated proteins, 14-3-3 epsilon can modulate activity, stability, and interactions, thereby influencing critical signaling pathways. Dysregulation of 14-3-3 epsilon expression or function can lead to a range of pathological conditions, including cancer, underscoring 14-3-3 epsilon′s importance in maintaining cellular homeostasis.

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14-3-3 ε Antibody (8C3) References:

1. The 4.1/ezrin/radixin/moesin domain of the DAL-1/Protein 4.1B tumour suppressor interacts with 14-3-3 proteins.  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
2. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1.  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
3. The 14-3-3 cancer connection.  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
4. Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions.  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
5. The protein YWHAE (14-3-3 epsilon) in spermatozoa is essential for male fertility.  |  Eisa, A., et al. 2021. Andrology. 9: 312-328. PMID: 32657535
6. 14-3-3 epsilon is an intracellular component of TNFR2 receptor complex and its activation protects against osteoarthritis.  |  Fu, W., et al. 2021. Ann Rheum Dis. 80: 1615-1627. PMID: 34226187
7. Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.  |  Jia, Y., et al. 2022. Cell Discov. 8: 21. PMID: 35228523
8. Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy.  |  Guo, M., et al. 2023. Cancer Biol Ther. 24: 2246203. PMID: 37599448
9. 14-3-3: modulators of signaling proteins?  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
10. Structural organization and chromosomal assignment of the human 14-3-3 eta chain gene (YWHAH).  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
11. The structural basis for 14-3-3:phosphopeptide binding specificity.  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
12. A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein.  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-14-3-3 ε Antibody (8C3) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-14-3-3 ε Antibody (8C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

14-3-3 ε Antibody (8C3) Specifications

Alternate Product Name: 14-3-3 epsilon

Product Clone Name: 8C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 136-152

Epitope Target: 14-3-3 ε

Epitope Species: ovine

Product Reactivity/Detection: 14-3-3 ε

Species Reactivity/Detection: mouse, rat, human, ovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for 14-3-3 ε Antibody (8C3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred 14-3-3 ε antibody is 14-3-3 ε Antibody (F-3): sc-393177.