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Puromycin dihydrochloride: sc-108071 |
| 说明书 | MSDS |
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- 产品规格
- 提供为 25 mg (sc-108071) 和 50 mg (sc-108071A) 尺寸和 250 mg (sc-108071B) 尺寸
- CAS号: 58-58-2
- 分子量: 544.5
- 分子式: C22H29N7O5.2HCl
- 纯度: >98%
- 物理外表: White to off-white powder
- 可溶性: Soluble in water (50 mg/ml)
- 简化分子线性输入规范 （SMILES): Cl.Cl.COc1ccc(cc1)CC(N)C(=O)N[C@@H]1[C@@H](CO)O[C@
H]([C@@H]1O)n1cnc2c(ncnc1 2)N(C)C
- 介绍
- Puromycin 是用于选择和维护细胞株表达一个转染的 pac 基因的一种氨基核酸酶抗生素，该基因的产物嘌呤- N -乙酰转移通过乙酰化将 Puromycin 灭活。
该产品已做细胞培养测试，推荐用于选择被圣克鲁斯生物技术公司的 shRNA 和慢病毒颗粒转染后的稳定转染细胞。
背景：Puromycin 是由土壤放线菌白色链球菌产生的一种抑菌物质，可在细胞内通过干扰 RNA 的功能来诱导细胞凋亡，从而抑制蛋白质合成。对真核和原核细胞都有毒性。用作可导致肽链过早终止的酰基-tRNA 类似物，并可阻滞 G2/M 期的细胞。
- 存储
- 使用
- For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, proceed with puromycin selection as follows:
NOTE: The working puromycin concentration for selection in mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line (see below). Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection.
• 48 hours post-transfection, aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration.
• Approximately every 2-3 days, aspirate and replace with freshly prepared selective media.
• Monitor the cells daily. Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days.
• Assay transfected cells.
NOTE: Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated.
Titrating puromycin for selecting transfected cell lines:
• Plate 2 x 105 cells in each well of a 6-well plate containing 3 ml of the appropriate complete medium plus increasing concentrations of puromycin (i.e., 0, 1.0, 2.5, 5.0, 7.5, and 10.0 µg/ml)
• Replace with fresh selective medium after 2 days to remove dead cells.
• Examine the wells for viable cells every two days.
• Monitor the cells daily and observe the percentage of surviving cells. Optimum effectiveness should be reached in 1-4 days.
• The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection.
- 安全
- 风险陈述: R36,R37,R38
- 安全陈述: S36,S37,S39
- 仅用于科研
- The pharmacological and toxicological properties of this product have not been fully investigated. Exercise caution in use and handling. This product must not be used in humans.
- 参考编号
- PubChem 数据库化合物识别编号: 64806
- 参考文献
- 1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187
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