BrdU Antibodies

PRODUCT NAME	CATALOG #	ISOTYPE	EPITOPE	APPLICATIONS	SPECIES
BrdU (2B1) Antibody	sc-56255	mouse IgG₁	N/A	IF, IHC(P), FCM	N/A
BrdU (3H579) Antibody	sc-70441	rat IgG_2a	N/A	IF, IHC(P), FCM	N/A
BrdU (BU1/75 ICR1) Antibody	sc-56258	rat IgG_2a	N/A	IF, IHC(P), FCM	N/A
BrdU (IIB5) Antibody	sc-32323	mouse IgG₁	N/A	IF, IHC(P), FCM
BrdU (3G26) Antibody	sc-70443	mouse IgG₁	N/A	IF, IHC(P), FCM	human
BrdU (5G15) Antibody	sc-70440	mouse IgG₁	N/A	IF, IHC(P), FCM	N/A
BrdU (5J234) Antibody	sc-70442	mouse IgG₁	N/A	FCM	N/A
BrdU (B33.1) Antibody	sc-56256	mouse IgG₁	N/A	IF, IHC(P), FCM	N/A
BrdU (Bu20A) Antibody	sc-20045	mouse IgG₁	N/A	FCM
BrdU (BU6-4) Antibody	sc-56259	mouse IgG₁	N/A	WB, FCM	N/A
BrdU (MBDU-65A) Antibody	sc-65723	mouse IgG₁	N/A	ELISA	N/A
BrdU (MoBU-1) Antibody	sc-51514	mouse IgG₁	N/A	IF, IHC(P), FCM	N/A
BrdU (SPM166) Antibody	sc-52989	mouse IgG₁	N/A	IF, IHC(P), FCM	N/A

BACKGROUND
The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdU immunodetection.