Post Transcriptional Gene Silencers

Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes. siRNAs are provided as pools of 3 target specific 19-25 nt siRNAs designed to knock down expression of specific gene of interest. Each vial contains 3 nmol of lyophilized siRNA and is sufficient for 50 - 100 transfections. For independent verification of gene silencing results, we also will provide, upon request, individual siRNA duplex components, each containing 3 nmol of lyophilized siRNA. shRNA Plasmid DNA Gene Silencers are provided as pools of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNA (sequences correspond to our well-established existing product line of siRNA gene silencers). shRNA Plasmids are provided as 20 µg transfection-ready plasmid DNA for either transient or stable (puromycin-selection) target specific gene silencing. shRNA Lentiviral Particles are provided as pools of three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin loop) shRNA and are provided as 200 µl transduction-ready viral particles for gene silencing in mammalian cells (human or mouse). Suitable control antibodies and RT-PCR primers are available for monitoring gene expression knockdown.