Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319): sc-58607

AChRα7 Antibody (319) is a rat monoclonal IgG1 antibody that detects AChRα7 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). AChRα7 Antibody (319) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AChRα7, also known as NACHRA7, CHRNA7-2, or CHRNA7, is a critical component of the nicotinic acetylcholine receptor family, which plays a vital role in fast synaptic transmission. AChRα7 is a 502 amino acid multi-pass membrane protein that forms a homopentamer, allowing creation of a transmembrane aqueous pore essential for ion flow. AChRα7 is predominantly located at the postsynaptic membrane of the neuromuscular junction, where binding acetylcholine facilitates ion influx leading to muscle contraction. Proper functioning of AChRα7 is crucial for neuromuscular signaling, and dysregulation has been implicated in various neurological disorders. AChRα7 interacts with RIC-3, a nicotinic acetylcholine receptor-associated protein, which is important for proper folding and trafficking of the receptor, further underscoring AChRα7′s significance in maintaining synaptic integrity and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319) References:

1. Probing conformational changes in the nicotinic acetylcholine receptor by Fourier transform infrared difference spectroscopy.  |  Baenziger, JE., et al. 1992. Biophys J. 62: 64-6. PMID: 1600101
2. Ligand-gated ion channels in the brain: the amino acid receptor superfamily.  |  Betz, H. 1990. Neuron. 5: 383-92. PMID: 1698394
3. Acetylcholinesterase reactivators modify the functional properties of the nicotinic acetylcholine receptor ion channel.  |  Alkondon, M., et al. 1988. J Pharmacol Exp Ther. 245: 543-56. PMID: 2452874
4. Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus.  |  Yang, NN., et al. 2021. Theranostics. 11: 4078-4089. PMID: 33754049
5. Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress.  |  Zhao, J., et al. 2023. Redox Biol. 59: 102594. PMID: 36603528
6. Anti-neuroinflammation effects of transcutaneous auricular vagus nerve stimulation against depression-like behaviors via hypothalamic α7nAchR/JAK2/STAT3/NF-κB pathway in rats exposed to chronic unpredictable mild stress.  |  Chen, Y., et al. 2023. CNS Neurosci Ther. 29: 2634-2644. PMID: 37032645
7. α7nAChR activation in AT2 cells promotes alveolar regeneration through WNT7B signaling in acute lung injury.  |  Chen, X., et al. 2023. JCI Insight. 8: PMID: 37410546
8. Neurotransmitter action: opening of ligand-gated ion channels.  |  Unwin, N. 1993. Cell. 72 Suppl: 31-41. PMID: 7679054
9. The diversity of neuronal nicotinic acetylcholine receptors.  |  Sargent, PB. 1993. Annu Rev Neurosci. 16: 403-43. PMID: 7681637
10. Quantal release of neurotransmitter and long-term potentiation.  |  Stevens, CF. 1993. Cell. 72 Suppl: 55-63. PMID: 8094037
11. The role of NMDA receptors in information processing.  |  Daw, NW., et al. 1993. Annu Rev Neurosci. 16: 207-22. PMID: 8460891
12. Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.  |  Ramirez-Latorre, J., et al. 1996. Nature. 380: 347-51. PMID: 8598930

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319) Specifications

Alternate Product Name: CHRNA7

Product Clone Name: 319

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: AChRα7

Epitope Species: chicken

Product Reactivity/Detection: both native and denatured nicotinic AChRα7

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin