shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
as sequencias de shRNA correspondem a MAGE-A siRNA sequencias de silenciadores geneticos
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
as sequencias de shRNA correspondem a MAGE-A siRNA siRNA Gene Silencer sequences
Apos a transduccao, linhas de celulas estaveis que expressam o shRNA em questao podem ser isoladas via selecao atraves da puromicina
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
