adenosine deaminase Antibody (D-4): sc-28346

Adenosine deaminase Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects adenosine deaminase of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-adenosine deaminase antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Adenosine deaminase plays a crucial role in the purine salvage pathway by catalyzing the deamination of adenosine to inosine, which is vital for regulating extracellular adenosine levels. Elevated adenosine can be toxic to lymphocytes, and thus, adenosine deaminase activity is essential for maintaining immune homeostasis. Additionally, adenosine deaminase interacts with CD26, influencing co-stimulatory signals in T cells, which is critical for effective immune responses. A deficiency in adenosine deaminase can lead to severe immunodeficiency diseases, such as severe combined immunodeficiency (SCID), and has been linked to hereditary hemolytic anemia, where adenosine deaminase levels can increase dramatically.

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adenosine deaminase Antibody (D-4) References:

1. Adenosine deaminase: functional implications and different classes of inhibitors.  |  Cristalli, G., et al. 2001. Med Res Rev. 21: 105-28. PMID: 11223861
2. A3 adenosine receptor signaling contributes to airway inflammation and mucus production in adenosine deaminase-deficient mice.  |  Young, HW., et al. 2004. J Immunol. 173: 1380-9. PMID: 15240734
3. The roles of adenosine deaminase in autoimmune diseases.  |  Gao, ZW., et al. 2021. Autoimmun Rev. 20: 102709. PMID: 33197575
4. Adenosine, adenosine- deaminase and implantable recorder outcome in syncopal patients.  |  Mitro, P., et al. 2022. Pacing Clin Electrophysiol. 45: 768-772. PMID: 35502914
5. Balancing early detection of adenosine deaminase deficiency with overdiagnosis risk.  |  Revak, S., et al. 2022. Ann Allergy Asthma Immunol. 129: 665-666. PMID: 36464395
6. Role of adenosine deaminase in prostate cancer progression.  |  Charles, C., et al. 2023. Am J Clin Exp Urol. 11: 594-612. PMID: 38148936
7. Analysis of normal and mutant forms of human adenosine deaminase - a review.  |  Daddona, PE. and Kelley, WN. 1980. Mol Cell Biochem. 29: 91-101. PMID: 6988697
8. Molecular basis of erythroenzymopathies associated with hereditary hemolytic anemia: tabulation of mutant enzymes.  |  Miwa, S. and Fujii, H. 1996. Am J Hematol. 51: 122-32. PMID: 8579052
9. SCID: the role of adenosine deaminase deficiency.  |  Resta, R. and Thompson, LF. 1997. Immunol Today. 18: 371-4. PMID: 9267078
10. Determination of adenosine deaminase binding domain on CD26 and its immunoregulatory effect on T cell activation.  |  Dong, RP., et al. 1997. J Immunol. 159: 6070-6. PMID: 9550406
11. Enzymatic and extraenzymatic role of ecto-adenosine deaminase in lymphocytes.  |  Franco, R., et al. 1998. Immunol Rev. 161: 27-42. PMID: 9553762
12. The structure and function of CD26 in the T-cell immune response.  |  Morimoto, C. and Schlossman, SF. 1998. Immunol Rev. 161: 55-70. PMID: 9553764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-adenosine deaminase Antibody (D-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-adenosine deaminase Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

adenosine deaminase Antibody (D-4) Specifications

Alternate Product Name: adenosine deaminase

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 64-363

Epitope Target: adenosine deaminase

Epitope Species: human

Product Reactivity/Detection: adenosine deaminase

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for adenosine deaminase Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin