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- mouse monoclonal IgM; 200 µg/ml
- also designated CD15
- raised against X-irradiated F9 teratocarcinoma stem cells
- recommended for detection of SSEA-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for immunoprecipitation, SSEA-1 (480) AC: sc-21702 AC, 500 µg/0.25 ml
- fluorescein (sc-21702 FITC), phycoerythrin (sc-21702 PE), PerCP (sc-21702 PerCP) and PerCP-Cy5.5 (sc-21702 PCPC5) conjugates for FCM, 100 tests
- Alexa Fluor® 405 (sc-21702 AF405), Alexa Fluor® 488 (sc-21702 AF488) and Alexa Fluor® 647 (sc-21702 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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SSEA-1 Background Information
Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro, while maintaining the potential to differentiate into derivatives of all three embryonic germ layers (1). Undifferentiated human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas (2) and are characterized by the expression of Stage Specific Embryonic Antigens SSEA-1and SSEA-3, TRA-2-39, TRA-2-54 and the high molecular weight glycoproteins TRA-1-60 and TRA-1-81 (3). In addition, SSEA-1, SSEA-3 and SSEA-4 are markers that characterize embryonic stem (ES) and embryonic germ (EG) cells (4). Specifically, undifferentiated cells from the human ES cell line H7 express SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, but not SSEA-1 (5). Interferon induces expression of SSEA-3 and SSEA-4 in EC cells without inhibiting their growth or inducing their differentiation (2).
| SSEA-1 (480) Citacoes dos Produtos |
Veja como outros autores utilizaram o anticorpo e/ou o anticorpo cojugado
18 total citations Citacoes
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SSEA-1 (480)
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SSEA-1 (480): sc-21702. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue (A) and immunofluorescence staining of methanol-fixed F9 cells (B) showing membrane localization.
SSEA-1 (480): sc-21702. Western blot analysis of SSEA-1 expression in mouse kidney tissue extract.
SSEA-1 (480) PE: sc-21702 PE. FCM analysis of human peripheral blood leukocytes (A) and NTERA-2 cl.D1 cells (B) showing specificity of SSEA-1 (480) for differentiated cells. Black line histogram represents the isotype control, normal mouse IgM: sc-2870. SSEA-1 (480) PE: sc-21702 PE. FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgM: sc-2870. SSEA-1 (480) Alexa Fluor 488: sc-21702 AF488. FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgM: sc-45079. SSEA-1 (480): sc-21702. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane and cytoplasmic staining of cells in tubules (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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