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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-1616-R
- epitope mapping at the C-terminus of Actin of human origin
- recommended for detection of a broad range of Actin isoforms of mouse, rat, human, zebrafish, C. elegans, Drosophila, and Xenopus origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-1616 P
- agarose conjugate for IP studies, sc-1616 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-1616 FITC) and phycoerythrin (sc-1616 PE) conjugates for intracellular FCM, 100 tests
- biotin conjugate, sc-1616 B, 200 µg/ml
- HRP conjugate, sc-1616 HRP, 200 µg/ml
- rhodamine (sc-1616 TRITC), conjugate for IHC, 200 µg/1 ml.
- Alexa Fluor® 405 (sc-1616 AF405), Alexa Fluor® 488 (sc-1616 AF488) and Alexa Fluor® 647 (sc-1616 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Actin Background Information
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. å-Actin expression is limited to various types of muscle, whereas -∫ and ©-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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Actin (I-19)
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Actin (I-19): sc-1616. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal fluorescein immunostaining of actin filaments. Note nuclear rhodamine immunostaining with PCNA (PC-10): sc-56. Actin (I-19): sc-1616. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.
Actin (I-19): sc-1616. Western blot analysis of Actin expression in C32 (A), BC3H1 (B) and Sol8 (C) whole cell lysates.
Actin (I-19) Alexa Fluor 488: sc-1616 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-45067. Actin (I-19) Alexa Fluor 647: sc-1616 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-45066. Actin (I-19): sc-1616. Western blot analysis of Actin expression in A-10 whole cell lysate.
Actin (I-19): sc-1616. Western blot analysis of ACTA1 expression in non-transfected: sc-117752 (A) and mouse ACTA1 transfected: sc-118228 (B) 293T whole cell lysates. Actin (I-19): sc-1616. Western blot analysis of ACTG2 expression in non-transfected: sc-117752 (A) and mouse ACTG2 transfected: sc-118229 (B) 293T whole cell lysates. Actin (I-19): sc-1616. Western blot analysis of γ-Actin expression in non-transfected 293T: sc-117752 (A), human γ-Actin transfected 293T: sc-116428 (B) and HeLa (C) whole cell lysates. Actin (I-19): sc-1616. Western blot analysis of ACTG1 expression in non-transfected 293T: sc-117752 (A), mouse ACTG1 transfected 293T: sc-124922 (B), HeLa (C) and A-431(D) whole cell lysates. Actin (I-19): sc-1616. Western blot analysis of β-Actin expression in non-transfected 293T: sc-117752 (A), human β-Actin transfected 293T: sc-111134 (B) and C32 (C) whole cell lysates. Actin (I-19): sc-1616. Western blot analysis of γ-Actin-2 expression in non-transfected 293T: sc-117752 (A), human γ-Actin-2 transfected 293T: sc-176378 (B) and C32 (C) whole cell lysates. Actin (I-19) FITC: sc-1616 FITC. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal Goat IgG: sc-3988. Actin (I-19) PE: sc-1616 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992. |
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