p-53BP1 Antibody (38.Ser 25): sc-135748

p-53BP1 Antibody (38.Ser 25) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-53BP1 of mouse and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-53BP1 (38.Ser 25) antibody is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). p-53BP1, also known as TP53BP1 or tumor protein p53 binding protein 1, is a crucial 1,972 amino acid protein that plays a significant role in cellular response to DNA damage. p-53BP1 is primarily located in the nucleus, where p-53BP1 associates with kinetochores and is involved in cell cycle regulation, particularly during mitosis. Two BRCT domains in p-53BP1 structure allow interaction with other proteins, including p53, thereby enhancing p53-mediated transcriptional activation and contributing to checkpoint signaling. This interaction maintains genomic stability by helping cells respond appropriately to DNA damage, preventing mutation propagation that could lead to cancer. p-53BP1 undergoes post-translational modifications, including phosphorylation at specific serine residues, which can influence activity and interactions. The gene encoding p-53BP1 is located on human chromosome 15, a region containing over 700 genes and representing nearly 3% of the human genome, underscoring p-53BP1 importance in cellular processes and potential implications in cancer biology.

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p-53BP1 Antibody (38.Ser 25) References:

1. 53BP1, a mediator of the DNA damage checkpoint.  |  Wang, B., et al. 2002. Science. 298: 1435-8. PMID: 12364621
2. 53BP1 functions in an ATM-dependent checkpoint pathway that is constitutively activated in human cancer.  |  DiTullio, RA., et al. 2002. Nat Cell Biol. 4: 998-1002. PMID: 12447382
3. 53BP1 links DNA damage-response pathways to immunoglobulin heavy chain class-switch recombination.  |  Manis, JP., et al. 2004. Nat Immunol. 5: 481-7. PMID: 15077110
4. 53BP1 facilitates long-range DNA end-joining during V(D)J recombination.  |  Difilippantonio, S., et al. 2008. Nature. 456: 529-33. PMID: 18931658
5. 53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility.  |  Dimitrova, N., et al. 2008. Nature. 456: 524-8. PMID: 18931659
6. Protein phosphatase 5 regulates the function of 53BP1 after neocarzinostatin-induced DNA damage.  |  Kang, Y., et al. 2009. J Biol Chem. 284: 9845-53. PMID: 19176521
7. Discordance between phosphorylation and recruitment of 53BP1 in response to DNA double-strand breaks.  |  Harding, SM. and Bristow, RG. 2012. Cell Cycle. 11: 1432-44. PMID: 22421153
8. AMPK-mediated phosphorylation on 53BP1 promotes c-NHEJ.  |  Jiang, Y., et al. 2021. Cell Rep. 34: 108713. PMID: 33596428
9. Phosphorylation of the DNA damage repair factor 53BP1 by ATM kinase controls neurodevelopmental programs in cortical brain organoids.  |  Lim, B., et al. 2024. PLoS Biol. 22: e3002760. PMID: 39226322
10. Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.  |  Iwabuchi, K., et al. 1998. J Biol Chem. 273: 26061-8. PMID: 9748285

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-53BP1 Antibody (38.Ser 25) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-53BP1 Antibody (38.Ser 25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-53BP1 Antibody (38.Ser 25) Specifications

Alternate Product Name: 53BP1

Product Clone Name: 38.Ser 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 25

Epitope Target: 53BP1

Epitope Species: mouse

Product Reactivity/Detection: Ser 25 phosphorylated 53BP1

Species Reactivity/Detection: mouse, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-53BP1 Antibody (38.Ser 25): AC: Agarose, HRP: Horseradish Peroxidase