YB-1 Antibody (59-Q): sc-101198

YB-1 Antibody (59-Q) is a mouse monoclonal IgG2a kappa light chain antibody that detects YB-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). YB-1 (59-Q) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. Y-box binding protein YB-1, also known as CCAAT-binding transcription factor, enhancer factor I subunit A, and DNA-binding protein B, is a multifunctional protein that plays a crucial role in regulating both transcription and translation. YB-1 interacts with diverse nucleic acid structures, functioning as a transcription factor and mRNA masking protein. YB-1′s modular structure, which includes a highly conserved N-terminal cold-shock domain and four basic C-terminal domains rich in Arginine clusters and aromatic residues, is essential for multifunctionality. This structural complexity allows YB-1 to modulate gene expression effectively, influencing cell proliferation by activating growth-associated genes while simultaneously repressing cell death-associated genes such as FAS. By regulating the balance between cell growth and apoptosis, YB-1 plays a pivotal role in maintaining cellular homeostasis and may be a critical factor in cancer biology, making YB-1 an important target for research in cell survival mechanisms.

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YB-1 Antibody (59-Q) References:

1. Regulation of the human fas promoter by YB-1, Puralpha and AP-1 transcription factors.  |  Lasham, A., et al. 2000. Gene. 252: 1-13. PMID: 10903433
2. Pleiotropic resistance to DNA-interactive drugs is associated with increased expression of genes involved in DNA replication, repair, and stress response.  |  Levenson, VV., et al. 2000. Cancer Res. 60: 5027-30. PMID: 11016623
3. Acquisition of double-stranded DNA-binding ability in a hybrid protein between Escherichia coli CspA and the cold shock domain of human YB-1.  |  Wang, N., et al. 2000. Mol Microbiol. 38: 526-34. PMID: 11069676
4. Cold shock domain factors activate the granulocyte-macrophage colony-stimulating factor promoter in stimulated Jurkat T cells.  |  Diamond, P., et al. 2001. J Biol Chem. 276: 7943-51. PMID: 11116154
5. Direct interaction of p53 with the Y-box binding protein, YB-1: a mechanism for regulation of human gene expression.  |  Okamoto, T., et al. 2000. Oncogene. 19: 6194-202. PMID: 11175333
6. Oncogenic TLS/ERG and EWS/Fli-1 fusion proteins inhibit RNA splicing mediated by YB-1 protein.  |  Chansky, HA., et al. 2001. Cancer Res. 61: 3586-90. PMID: 11325824
7. Y-box binding protein 1--a prognostic marker and target in tumour therapy.  |  Kosnopfel, C., et al. 2014. Eur J Cell Biol. 93: 61-70. PMID: 24461929
8. Y box binding protein 1 inhibition as a targeted therapy for ovarian cancer.  |  Tailor, D., et al. 2021. Cell Chem Biol. 28: 1206-1220.e6. PMID: 33713600
9. Targeting the Y-box Binding Protein-1 Axis to Overcome Radiochemotherapy Resistance in Solid Tumors.  |  Lettau, K., et al. 2021. Int J Radiat Oncol Biol Phys. 111: 1072-1087. PMID: 34166770
10. YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer.  |  Jiang, D., et al. 2022. Cell Death Differ. 29: 1283-1295. PMID: 35022570
11. Upregulated YB-1 protein promotes glioblastoma growth through a YB-1/CCT4/mLST8/mTOR pathway.  |  Wang, JZ., et al. 2022. J Clin Invest. 132: PMID: 35239512
12. Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos.  |  Jiang, WJ., et al. 2023. Front Cell Dev Biol. 11: 1238546. PMID: 37965572

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-YB-1 Antibody (59-Q) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-YB-1 Antibody (59-Q) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

YB-1 Antibody (59-Q) Specifications

Alternate Product Name: YBX1

Product Clone Name: 59-Q

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 51-140

Epitope Target: YB-1

Epitope Species: human

Product Reactivity/Detection: YB-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for YB-1 Antibody (59-Q): N/A