epitope corresponding to a short amino acid sequence containing Thr 71 phosphorylated ATF-2 of human origin
recommended for detection of ATF-2 phosphorylated at Thr 71 and correspondingly phosphorylated ATF-7 of m, r and h origin by WB, IP, IF, IHC(P), FCM and ELISA
blocking peptide, sc-8398 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-8398 X, 200 µg/0.1 ml
agarose conjugate for IP studies, sc-8398 AC, 500 µg/0.25 ml agarose
fluorescein (sc-8398 FITC) and rhodamine (sc-8398 TRITC) conjugates for immunofluorescence, 200 µg/ml
for phosphorylation positive (sc-2247) and non-activated control (sc-2210) cell extracts
Alexa Fluor® 405 (sc-8398 AF405), Alexa Fluor® 488 (sc-8398 AF488) and Alexa Fluor® 647 (sc-8398 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
Mayer, SI. et al. 2008. Expression of the transcriptional repressor ATF3 in gonadotrophs is regulated by Egr-1, CREB, and ATF2 after gonadotropin-releasing hormone receptor stimulation. Endocrinology. 149: 6311-6325. View PubMed Entry
Reimer, T. et al. 2007. Type I IFN Induction in Response to Listeria monocytogenes in Human Macrophages: Evidence for a Differential Activation of IFN Regulatory Factor 3 (IRF3). J. Immunology. 179: 1166-1177. View PubMed Entry
Ozer, A. et al. 2005. The candidate tumor suppressor ING4 represses activation of the hypoxia inducible factor (HIF). Proc. Natl. Acad. Sci. USA. 102(21): 7481-7486. View PubMed Entry
Lewis, J.S. et al. 2005. Differential effects of 16alpha-hydroxyestrone and 2-methoxyestradiol on cyclin D1 involving the transcription factor ATF-2 in MCF-7 breast cancer cells. J Mol Endocrinol. 34: 91-105. View PubMed Entry
Kamiguti, A.S. et al. 2003. Regulation of hairy-cell survival through constitutive activation of mitogen-activated protein kinase pathways. Oncogene. 22: 2272-2284. View PubMed Entry
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
p-ATF-2 (F-1) Data
Click on image to enlarge
Western blot analysis of ATF-2 activation in untreated (A,C) and anisomycin-induced (B,D) NIH/3T3 cells. Antibodies tested include a phospho-specific ATF-2 monoclonal, p-ATF-2 (F-1): sc-8398 (A,B) and a control antibody, ATF-2 (N-96): sc-6233 (C,D).
p-ATF-2 (F-1): sc-8398. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of activated ATF-2.
