Western blot analysis of Akt1/2/3 phosphorylation in untreated (A, D), calyculin treated (B, E) and calyculin and lambda protein phosphatase treated (C, F) Jurkat whole cell lysates. Antibodies tested include p-Akt1/2/3 (Ser 473):
sc-33437 (A, B, C) and Akt1 (C-20):
sc-1618 (D, E, F).