Western blot analysis of Akt1/2/3 phosphorylation in untreated (A, D), insulin treated (B, E) and insulin and lambda protein phosphatase treated (C, F) 293T whole cell lysates. Antibodies tested include p-Akt1/2/3 (Thr 308)-R:
sc-16646-R (A, B, C) and Akt1/2/3 (H-136):
sc-8312 (D, E, F).