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CTCF Antibody (C-20): sc-15914

 |  Datasheet

(Based on data analysis)

  • CTCF Antibody (C-20) is a goat polyclonal IgG provided at 200 µg/ml
  • epitope mapping near the C-terminus of CTCF of human origin
  • recommended for detection of CTCF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • blocking peptide, sc-15914 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-15914 X, 200 µg/0.1 ml
  • See 11 citations for CTCF Antibody (C-20)
 

See additional CTCF Antibodies.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
CTCF Antibody (C-20) sc-15914 200 µg/ml $279
CTCF (C-20) P sc-15914 P
(peptide)
100 µg/0.5 ml $61
CTCF Antibody (C-20) X sc-15914 X 200 µg/0.1 ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CTCF CRISPR/Cas9 KO Plasmid (h) sc-401245 20 µg $345
CTCF HDR Plasmid (h) sc-401245-HDR 20 µg $385
CTCF CRISPR/Cas9 KO Plasmid (m) sc-419869 20 µg $345
CTCF HDR Plasmid (m) sc-419869-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CTCF siRNA (h) sc-35124 10 µM $258
CTCF siRNA (m) sc-35125 10 µM $258
CTCF (h)-PR sc-35124-PR 10 µM, 20 µl $23
CTCF (m)-PR sc-35125-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CTCF shRNA Plasmid (h) sc-35124-SH 20 µg $520
CTCF shRNA Plasmid (m) sc-35125-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CTCF shRNA (h) Lentiviral Particles sc-35124-V 200 µl $625
CTCF shRNA (m) Lentiviral Particles sc-35125-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jurkat nuclear extract sc-2132 250 µg/0.05 ml $143
K-562 nuclear extract sc-2130 250 µg/0.05 ml $143
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
CCRF-CEM nuclear extract sc-2146 250 µg/0.05 ml $143
CTCF (m2): 293T Lysate sc-119494 100 µg/200 µl $205
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
CTCF (h): 293T Lysate sc-170327 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CTCF 10664 16q22.1 NM_006565 P49711
604167
Mouse Ctcf 13018 8 D3 Q61164
N/A
 


CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression.

CTCF Antibody (C-20) Product Citations
See how others have used CTCF Antibody (C-20) and or CTCF Antibody (C-20) conjugates.


11 total citations
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CTCF Antibody (C-20) Data
Click on image to enlarge
CTCF siRNA (h): sc-35124. Western blot analysis of CTCF expression in non-transfected control (A) and CTCF siRNA transfected (B) HeLa cells. Blot probed with CTCF (C-20): sc-15914. α-actinin (H-2): sc-17829 used as specificity and loading control.
CTCF (C-20): sc-15914. Western blot analysis of CTCF expression in CCRF-CEM nuclear extract.
CTCF (C-20): sc-15914. Western blot analysis of CTCF expression in non-transfected: sc-117752 (A) and mouse CTCF transfected: sc-119494 (B) 293T whole cell lysates.
CTCF (C-20): sc-15914. Western blot analysis of CTCF expression in non-transfected: sc-117752 (A) and human CTCF transfected: sc-170327 (B) 293T whole cell lysates and Jurkat nuclear extract (C).
CTCF (C-20): sc-15914. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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