Western blot detection of CaMKII phosphorylation in mouse brain extracts. Blots were probed with CaMKII (M-176)-R:
sc-9035-R (A) and p-CaMKIIα (Thr 286)-R:
sc-12886-R (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively.