p-4E-BP1 (Ser 65/Thr 70) Antibody sc-12884

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p-4E-BP1 (Ser 65/Thr 70): sc-12884

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goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-12884-R
epitope corresponding to a short amino acid sequence containing phosphorylated Ser 65 and Thr 70 of 4E-BP1 of human origin
recommended for detection of Ser 65 and Thr 70 phosphorylated 4E-BP1 of m, r and h origin by WB, IP, IF and ELISA
blocking peptide, sc-12884 P

Additional 4E-BP1 Antibodies ...

Ordering Information Product Citations

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WB IP IF siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	EIF4EBP1	1978	8p12	NM_004095	Q13541	602223
Mouse	Eif4ebp1	13685	8 A2		Q60876	N/A

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Wish List
p-4E-BP1 (Ser 65/Thr 70)	sc-12884	200 µg/ml	$259
p-4E-BP1 (Ser 65/Thr 70) P	sc-12884 P (peptide)	100 µg/0.5 ml	$58
p-4E-BP1 (Ser 65/Thr 70)-R	sc-12884-R	200 µg/ml	$259

siRNA Gene Silencers (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
4E-BP1 siRNA (h)	sc-29594	10 µM	$245
4E-BP1 siRNA (m)	sc-29595	10 µM	$245
4E-BP1 (h)-PR	sc-29594-PR	10 µM	$22
4E-BP1 (m)-PR	sc-29595-PR	10 µM	$22

shRNA Plasmids (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
4E-BP1 shRNA Plasmid (h)	sc-29594-SH	20 µg	$495
4E-BP1 shRNA Plasmid (m)	sc-29595-SH	20 µg	$495

shRNA Lentiviral Particles (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
4E-BP1 shRNA (h) Lentiviral Particles	sc-29594-V	200 µl	$595
4E-BP1 shRNA (m) Lentiviral Particles	sc-29595-V	200 µl	$595

WB Positive Control Cell Lysates (click catalog # for product information)

Product Name	Catalog #	Unit	Price	Qty	Wish List
4E-BP1 (m): 293T Lysate	sc-118030	100µg/200µl	$195
4E-BP1 (h): 293T Lysate	sc-116590	100µg/200µl	$195
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$99

p-4E-BP1 (Ser 65/Thr 70) Product Citations

See how others have used p-4E-BP1 (Ser 65/Thr 70): sc-12884 antibody and or p-4E-BP1 (Ser 65/Thr 70) antibody conjugates.

Gong, J. et al. 2009. Serine/threonine kinase Pim-2 promotes liver tumorigenesis induction through mediating survival and preventing apoptosis of liver cell. J. Surg. Res. 153: 17-22. View PubMed Entry

Rizzieri, DA. et al. 2008. A phase 2 clinical trial of deforolimus (AP23573, MK-8669), a novel mammalian target of rapamycin inhibitor, in patients with relapsed or refractory hematologic malignancies. Clin. Cancer Res. 14: 2756-2762. View PubMed Entry

Zhang, Q. et al. 2007. Nicotine induces hypoxia-inducible factor-1alpha expression in human lung cancer cells via nicotinic acetylcholine receptor-mediated signaling pathways. Clin. Cancer Res. 13: 4686-4694. View PubMed Entry

Legrier, ME. et al. 2007. Targeting protein translation in human non small cell lung cancer via combined MEK and mammalian target of rapamycin suppression. Cancer Res. 67(23): 11300-11308. View PubMed Entry

p-4E-BP1 (Ser 65/Thr 70) Data

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p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of phosphorylated 4E-BP1 expression in untreated (A), insulin-treated (B) and serum starved + serum (C) NIH/3T3 whole cell lysates.

Western blot analysis of phosphorylated 4E-BP1 expression in untreated (A,D), insulin-treated (B,E) and serum starved + serum (C,F) NIH/3T3 whole cell lysates. Blots were probed with p-4E-BP1 (Ser 65/Thr 70): sc-12884 (A,B,C) and p-4E-BP1 (Ser 65/Thr 70): sc-12884 preincu-bated with its cognate phosphorylated peptide (D,E,F).

p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and mouse 4E-BP1 transfected: sc-118030 (B) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in A-431 whole cell lysate.

p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and mouse 4E-BP1 transfected: sc-118030 (B) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 phosphorylation in 293T (A) and HeLa (B) whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 expression in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1 (Ser 65/Thr 70): sc-12884 (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).

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