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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-805-G
- epitope mapping within an internal region of the influenza hemagglutinin (HA) protein
- recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA
- blocking peptide, sc-805 P
- agarose conjugate for IP studies, sc-805 AC, 500 µg/0.25 ml agarose
- Available as TransCruz reagent for ChIP applications, sc-805 X, 200 µg/0.1 ml
- biotin conjugate, sc-805 B, 200 µg/ml
- fluorescein (sc-805 FITC) and rhodamine (sc-805 TRITC) conjugates for immunofluorescence, 200 µg/ml
- HRP (sc-805 HRP), conjugate, 200 µg/ml
- Alexa Fluor® 405 (sc-805 AF405), Alexa Fluor® 488 (sc-805 AF488) and Alexa Fluor® 647 (sc-805 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Conditions GénéralesRéférences bibliographiques
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HA-probe Background Information
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
| HA-probe (Y-11) Références bibliographiques |
Consulter les citations pour ces anticorps HA-probe (Y-11): sc-805 et/ou un anticorps HA-probe (Y-11) conjugué.
655 total citations En chargeant des citations.
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trademark of Molecular Probes Inc., OR., USA |
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HA-probe (Y-11)
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HA-probe (Y-11): sc-805. Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.
Western blot analysis of HA epitope-tagged JNK2 (1-424): sc-4062. Antibodies tested include HA-probe (Y-11)-G: sc-805-G (A) and HA-probe (Y-11): sc-805 (B,C). HA-probe (Y-11)-G: sc-805-G. Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates. |
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